ERA technology
Enzymatic Recombinase Amplification (ERA ) Technology is a global leading and independent intellectual property isothermal nucleic acid amplification technology developed by GenDx Biotech. ERA employeed advanced Molecular Design, Directed Evolution, Affinity Mathematics and other techniques in the field of antibody pharmacy to transform and mutate specific enzymes from bacteria, viruses and bacteriophages. Following that, screening of functional mutants and optimizing of the combinations are conducted. The core recombination isothermal amplification system was obtained. The method was applied for Chinese patents and international patents 【ZL 2017 1 0317160.2，ZL 2018 1 0854104.7，ZL 2018 1 0505203.4，201910262085.3，202010160768，202010394538.0，202210193295.3 and PCT/2019/090586，PCT/2021/082213】. The method can specifically amplify trace target DNA fragments at a constant temperature between 30-45℃. At the optimal temperature of 35-45℃, the amplification process only takes 5-15 minutes. Its low temperature adaptability and sensitivity have made cutting-edge breakthroughs globally.
Technological advantages
1. Fast and easy to operate, from sample prossing to getting results in 15 minutes.
2. High sensitivity, capable of detecting as low as 1-10 copies.
3. Strong resistance to inhibitors, requiring only simple sample preparation before direct reaction and test.
4. Isothermal amplification, low equipment requirements, with an optimal temperature of 35℃-45℃, making it compatible with a wide range of devices.
5. Closed-tube reaction and detection to avoid aerosol contamination, ensuring high safety.
ERA provides a rapid, accurate, and convenient biological nucleic acid detection solution.
Comparison of techniques
