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Materials and methods

To detect 3C protease in lysate of coxsackievirus-infected HEK293T cells, lysates from 2 k cells were added to 50 μL of CRISPR  buffer and incubated at 37°C for 4.5 hours. The reaction solution was then added to the test strip's sample area, and the bands were  visually observed for about 10 minutes. The concentrations of other components in the 50 μL reaction system were 31 nM for CasPS3C,  40 nM for gRNA and dsDNA, 200 nM for ssDNA-FAM-Biotin reporter nucleic acid, and 200 μM for Mg2+. The CRISPR test strips used  in the study were purchased from Suzhou GenDx Biotech Technology Co., Ltd.