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In summary, to promote the accurate diagnosis of sus-pected cases in the early stage of infection, we developed a rapid, highly sensitive, convenient SARS-CoV-2 detection method that utilizes RT-ERA amplification and the CRISPR/Cas12a system. This testing system can be used for on-site SARS-CoV-2 detection with a sensitivity of 7.5 copies of SARS-CoV-2 N gene and 15 copies of SARS-CoV-2 ORF1ab gene per 30-lL reaction in 40 min. Finally, we simplified the requirements for on-site detection instruments by using the CRISPR/Cas12a method combined with immunochromatographic strips, resulting in a powerful and effective tool for on-site detection of SARS-CoV-2 with high sensitivity and low cost.