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Materials and methods:

Lumpy skin disease virus (LSDV) is a severe and highly contagious form of cowpox. As LSDV continues to mutate and there is no vaccine and treatment in nonendemic countries, early detection of LSDV becomes an important basis for epidemic prevention and control, especially for detection of conserved sequences. A new label-free and sensitive fluorescence method was developed based on a light-up RNA aptamer for detecting LSDV. The method integrated recombinase polymerase amplification (RPA), CRISPR/Cas12a, 10− 23 DNAzyme, and Baby Spinach RNA aptamer for triple cascade signal amplification.

The basic ERA kit as an RPA reagent was purchased from GenDx Biotech Co., Ltd.

Doi: org/10.1021/acs.analchem.4c00962