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Abstract

Feline calicivirus (FCV ) has a single-stranded, positive-sense RNA genome, and it is responsible for many infectious res.piratory diseases in cats. In addition, more worryingly, highly virulent strains of FCV can cause high mortality in felines[herefore, a rapid and reliable diagnosis tool plays an important role in controlling the outbreak of FCV, In this study, enzymatic recombinase amplification (ERA) assay combined with lateral fiow dipstick (LFl)) was developed for the detectionof FCy, targeting a relatively conversed position of FCV-0RF]. The results showed that the optimal reaction condition wasat 40 'C for 30 min., ERA-I FI method was highly sensitive with the detection limit as low as 3.2 TCID. Of FCV RNA peIreaction. The specificity analysis demonstrated no cross-reactivity with feline parvovirus (FpV), feline herpesvirus (FHV) andfelimne infectious peritonitis virus (FlPV). ER A-LFD was highly repeatable and reproducible, with the intra-assay and inter.assay coeficients of variation for this method both less than 7%. The general test showed that all the recombinant plasmidswith known mutant sites and FCV strains with diferent mutant sites stored in our laboratory were all detected by this methodOf the 23 samples, 14 samples were tested positive for FCV by ERA-LFI) and R'T-gPCR, respectively, In summary, ERA.IFD assay was a fast, accurate and convenient diagnosis tool for the detection of FCV.

Doi: org/10.1007/s00253-022-11785-6